Download DeconvDemo for free. An image deconvolution / deblur software. DeconvDemo is a Qt & opencv based image deconvolution/ deblur software which is including the spatial and frequency domain solver. Image deconvolution / deblur software with non-blind deconvolution algorithm New: Add pregressive non-blined deconvolution Add blind kernel estimation Implemented mehtod: METHODWIENER, METHOD. MassLynx Mass Spectrometry Software Waters’ MassLynx ™ software enhances your MS system thanks to an intuitive interface, intelligent instrument control and software features designed specifically for the most important element of the analysis: the sample. At the workplace, we use MassLynx, but I need to work from home with these data, and I need a free software to view and process chromatograms. Example of data file. MassLynx is a software package to control analytical equipment produced by Waters Corporation including liquid chromatography systems such as the ACQUITY UPLC series of UHPLC systems and mass spectrometers such as the Xevo TQ-S.
What drug discovery scientists view as the bottleneck in finding clinical candidates changes as synthetic and screening technologies leapfrog each other. At this point, combinatorial chemistry clearly leads early-stage screening methods in terms of throughput, a trend expected to continue for some time. So today there's a big premium on getting compounds through screens as fast as they are made.
Very early pharmacokinetic and ADME (absorption, distribution, metabolism, and excretion) studies have taken on a 'combinatorial' character of their own: In vitro methods are replacing animals; even when animals are used, preliminary studies use 'cassettes,' or panels of drugs administered simultaneously. Managing information from such studies requires software that reflects this complexity while accommodating high instrument sensitivity.
QuanLynx, a module now offered with Micromass's MassLynx software family, automates unattended multiple reaction monitoring (MRM) and selected ion monitoring (SRM), two screening methods enabled by modern mass spectrometry. MRM is typically carried out on complex tissue samples where animals have been subjected to a cassette, or panel, of discovery-stage compounds. Since such samples are complex and contain very low levels of drugs and metabolites, MRM is limited to triple quarupole or MS/MS instruments. SIR, sometimes referred to as the 'poor cousin' of MRM, is adequate for some single-drug experiments as well as in vitro work. SIR may be carried on a single quad MS. About 70% of preliminary drug screening requires triple quad instruments and thus uses the MRM protocol.
A view from the QuanLynx application wizard.
QuanLynx integrates four distinct processes:
LI>MS-MS optimization
Construction of acquisition method and data acquisition
Construction of quantification method and quantification
Data reporting
Micromass believes these capabilities will ultimately expedite high throughput ADME/PK screening assays of diverse discovery compounds. How to subway surfers on mac. 'Researchers at pharmaceutical companies have written their own macros that achieve similar automated methods development,' McDowall stated, 'but QuanLynx is the first professionally coded shrink-wrapped product to be introduced to the market for this explosively growing application.'
QuanLynx allows analysts to define an experimental process by selecting options from a logical sequence of dialogue boxes presented in a familiar Microsoft Windows NT environment. The software then automatically determines the optimum MS parameters for each compound before quantitation. QuanLynx can be implemented on Micromass's Quattro II, Quattro LC and the new Quattro Ultima tandem quadrupole instruments and on the Waters ZMD single quadrupole running under MassLynx 3.3 (or higher).
High-Throughput: The Name of the Game Because of a greater focus on the survival of drug candidates up to and beyond first testing in humans, stability, toxicity, adsorption, physiochemical properties, and other ADME/PK assays are being adapted to the high-throughput discovery environment. This would typically involve swiftly assaying hundreds or thousands of potential candidates against each other to optimize efficacy over a series. Generally, absolute measurements are less important than relative ones, high throughput being the ultimate goal. 'Very early screens involve testing multiple compounds from a combinatorial library, simultaneously, in the same animal,' says Micromass Marketing Director Mark A. McDowall. 'Since multiple drugs are being tested together, you need a means of finding interactions. What scientists are really looking for here are relative activities, rather than one big hit. Classic ADME/PK experiments can then be done later on. Like combichem, it's a game of big numbers.'
Waters' ZMD 2000 single quadrupole MS.
Those 'big numbers' are reduced somewhat by reducing the number of samples tested, an approach known as 'n-in-one' dosing, or by single compound dosing with subsequent sample pooling. By either method, the analytical bottleneck is the same—high-throughput quantitation of multiple compounds per sample at reduced concentration levels. One way to attain maximum throughput in this environment is to use efficient, generic HPLC methods. In high performance LC-MS/MS, however, it is not possible to operate with generic MS/MS parameters. QuanLynx eliminates what were traditionally the rate-limiting steps in developing optimized MS parameters for each compound of interest and setting up quantification methods.
Today's ADMD/PK screening involves high throughput quantitative analysis of large numbers of discovery compounds, usually over a time course. Traditional approaches use HPLC-UV or radiolabeling studies. LC-MS is much more selective since it uses a mass signature to identify compounds for identification and subsequent unambiguous quantitation. Mixtures of compounds can therefore be studied in parallel provided that each compound in the cassette has a unique mass signature.
Inside the Quattro Ultima, Micromass's top-of-the-line triple quad instrument.
Waters Masslynx
'We expect QuanLynx tool will allow our customers to make substantial gains in throughput,' McDowall said. 'QuanLynx leverages the competitive position of our single quadrupole [Waters ZMD 2000], LC-TOF [Micromass LCT] and triple quadrupole [Micromass Quattro Ultima] mass spectrometers with respect to those from our major competitor [Perkin-Elmer] in this application.
Masslynx software, free downloads
QuanLynx is bundled at no additional cost with MassLynx 3.4 (available since August, 1999), MicroMass's established 32 bit Windows NT data handling system for LC-MS and LC-MS-MS.
For more information: Mark A. McDowall, International Marketing Manager, Micromass UK Ltd., Floats Road, Manchester M23 9LZ, UK. Tel: +44 (0) 161 945 4170. Fax: +44 (0) 161 998 8915.